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Deep, qualified subsequent-generation sequencing has unveiled that subclonal mutations (i.e., Those people existing in only a fraction of tumor cells) can be detected for all driver genes and are connected with speedy illness progression and weak consequence.11–thirteen This is particularly pertinent for TP53 mutations given The point that, as described down below, CLL therapy relies about the presence or absence of those mutations. The present consensus is usually that, aside from clonal mutations, subclonal mutations having a variant allelic frequency starting from five to 10% (and therefore underneath the edge of detection by traditional molecular procedures) could also be described, Whilst those by using a variant allelic frequency reduced than 5% mustn't, but there's A great deal controversy all over these issues which advice may change Down the road.
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